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MiR‐143‐3p regulates chondrogenic differentiation of synovium derived mesenchymal stem cells under mechanical stress through the BMPR2‐Smad signalling pathway by targeting BMPR2.
- Source :
- Journal of Oral Rehabilitation; Aug2024, Vol. 51 Issue 8, p1507-1520, 14p
- Publication Year :
- 2024
-
Abstract
- Background: Mesenchymal stem cells (MSCs) derived from the synovium, known as synovium mesenchymal stem cells (SMSCs), exhibit significant potential for articular cartilage regeneration owing to their capacity for chondrogenic differentiation. However, the microRNAs (miRNAs) governing this process and the associated mechanisms remain unclear. While mechanical stress positively influences chondrogenesis in MSCs, the miRNA‐mediated response of SMSCs to mechanical stimuli is not well understood. Objective: This study explores the miRNA‐driven mechano‐transduction in SMSCs chondrogenesis under mechanical stress. Methods: The surface phenotype of SMSCs was analysed by flow cytometry. Chondrogenesis capacities of SMSCs were examined by Alcian blue staining. High throughput sequencing was used to screen mechano‐sensitive miRNAs of SMSCs. The RNA expression level of COL2A1, ACAN, SOX9, BMPR2 and miR‐143‐3p of SMSCs were tested by quantitative real‐time polymerase chain reaction (qRT‐PCR). The interaction between miR‐143‐3p and TLR4 was confirmed by luciferase reporter assays. The protein expression levels of related genes were assessed by western blot. Results: High‐throughput sequencing revealed a notable reduction in miR‐143‐3p levels in mechanically stressed SMSCs. Gain‐ or loss‐of‐function strategies introduced by lentivirus demonstrated that miR‐143‐3p overexpression hindered chondrogenic differentiation, whereas its knockdown promoted this process. Bioinformatics scrutiny and luciferase reporter assays pinpointed a potential binding site for miR‐143‐3p within the 3′‐UTR of bone morphogenetic protein receptor type 2 (BMPR2). MiR‐143‐3p overexpression decreased BMPR2 expression and phosphorylated Smad1, 5 and 8 levels, while its inhibition activated BMPR2‐Smad pathway. Conclusion: This study elucidated that miR‐143‐3p negatively regulates SMSCs chondrogenic differentiation through the BMPR2‐Smad pathway under mechanical tensile stress. The direct targeting of BMPR2 by miR‐143‐3p established a novel dimension to our understanding of mechano‐transduction mechanism during SMSC chondrogenesis. This understanding is crucial for advancing strategies in articular cartilage regeneration. [ABSTRACT FROM AUTHOR]
- Subjects :
- FLOW cytometry
SYNOVIAL membranes
CARRIER proteins
TEMPOROMANDIBULAR joint
RESEARCH funding
MICRORNA
MESENCHYMAL stem cells
POLYMERASE chain reaction
TRANSCRIPTION factors
CELLULAR signal transduction
QUANTITATIVE research
CELL culture
CHONDROGENESIS
PHYSIOLOGIC strain
WESTERN immunoblotting
CELL differentiation
CELL receptors
Subjects
Details
- Language :
- English
- ISSN :
- 0305182X
- Volume :
- 51
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Journal of Oral Rehabilitation
- Publication Type :
- Academic Journal
- Accession number :
- 178427265
- Full Text :
- https://doi.org/10.1111/joor.13726