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Raspberry-shaped ZIF-8/Au nanozymes with excellent peroxidase-like activity for simple and visual detection of glutathione.

Authors :
Zhu, Zhentong
Wang, Xiaoli
Wang, Na
Zeng, Chaoqin
Zhang, Lei
Fan, Jiamin
Yang, Xin
Li, Peizhe
Yuan, Hongxia
Feng, Yanjun
Huo, Shuhui
Lu, Xiaoquan
Source :
Analytical & Bioanalytical Chemistry; Aug2024, Vol. 416 Issue 19, p4417-4426, 10p
Publication Year :
2024

Abstract

Artificial enzymes with high stability, adjustable catalytic activity, controllable preparation, and good reproducibility have been widely studied. Noble metal nanozymes, particularly gold nanoparticles (Au NPs), exhibit good catalytic activity, but their stability is poor. In this study, zeolitic imidazolate framework-8 (ZIF-8) was used as a carrier for Au NPs, thus improving the utilization efficiency and conservation stability of the nanozymes. A ZIF-8/Au nanocomposite with peroxidase activity and a raspberry-shaped structure was synthesized. In the assay, ZIF-8/Au catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue product oxidized TMB (oxTMB). Glutathione (GSH) selectively inhibited this reaction, with a detection limit of 0.28 µM and linear range of 0.5–60 µM. Using the photo and chromaticity analysis functions, we developed a portable analysis method using a smartphone equipped with a camera module as a detection terminal for a wide range of rapid screening techniques for GSH. Preparation of raspberry-shaped ZIF-8/Au improved the catalytic activity of Au NPs and good results were demonstrated in serum, which suggests their promising application under physiological conditions. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16182642
Volume :
416
Issue :
19
Database :
Complementary Index
Journal :
Analytical & Bioanalytical Chemistry
Publication Type :
Academic Journal
Accession number :
178530093
Full Text :
https://doi.org/10.1007/s00216-024-05378-7