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Utility of immunofixation in complementing and empowering serum protein electrophoresis in the diagnosis of paraproteinemia: Experience at a tertiary care center.
- Source :
- Medical Laboratory Journal; Mar/Apr2024, Vol. 18 Issue 2, p1-4, 4p
- Publication Year :
- 2024
-
Abstract
- Background: M’ proteins or paraproteins refer to immunoglobulins that are produced by clonal plasma cells and are a characteristic feature of monoclonal gammopathies. Routine electrophoresis on agarose gel and immunofixation can be used to detect immunoglobulin paraprotein (M-protein). We aimed to evaluate the performance of agarose gel electrophoresis alone and in combination with immunofixation for detecting serum M-proteins. Methods: One hundred and twenty-three patients suspected of paraproteinemia were evaluated. Routine serum protein electrophoresis (SPE) and immunofixation electrophoresis (IFE) protocols were performed. Data from SPE, and SPE-IFE (gel images and electrophoretograms) were collected and reviewed. Results: 21% cases were confirmed using the SPE-IFE combination, and among them, 33% had positive light chain (λ) only on IFE. Similarly, nine cases with biclonal gammopathy on SPE were characterized by IFE. Conclusion: IFE can be a confirmatory test in cases where SPE results are not reliable and it can be a complementary test when characterization of the M protein detected on SPE is required. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 25384449
- Volume :
- 18
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Medical Laboratory Journal
- Publication Type :
- Academic Journal
- Accession number :
- 178724618
- Full Text :
- https://doi.org/10.29252/mlj.18.2.1