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Metagenomic next-generation sequencing as a diagnostic tool in the clinical routine of an infectious diseases department: a retrospective cohort study.
- Source :
- Infection; Aug2024, Vol. 52 Issue 4, p1595-1600, 6p
- Publication Year :
- 2024
-
Abstract
- Background: Metagenomic next-generation sequencing (mNGS) of circulating cell-free DNA from plasma is a hypothesis-independent broadband diagnostic method for identification of potential pathogens. So far, it has only been investigated in special risk populations (e.g. patients with neutropenic fever). Purpose: To investigate the extent to which mNGS (DISQVER® platform) can be used in routine clinical practice. Methods: We collected whole blood specimens for mNGS testing, blood cultures (BC), and pathogen-specific PCR diagnostics. Clinical data and pathogen diagnostics were retrospectively reviewed by an infectious disease expert panel regarding the adjustment of anti-infective therapy. Results: In 55 selected patients (median age 53 years, 67% male) with heterogeneous diagnoses, a total of 66 different microorganisms and viruses were detected using mNGS (51% viruses, 38% bacteria, 8% fungi, 3% parasites). The overall positivity rate of mNGS was 53% (29/55). Fifty-two out of 66 (79%) potential pathogens detected by mNGS were found in patients with primary or secondary immunodeficiency. The concordance rates of BC and pathogen-specific PCR diagnostics with mNGS testing were 14% (4/28) and 36% (10/28), respectively (p < 0.001). An additional bacterial pathogen (Streptococcus agalactiae) could only be detected by BC. Therapeutic consequences regarding anti-infective therapy were drawn from 23 pathogens (35% of detections), with 18 of these detections occurring in patients with immunodeficiency. Conclusions: We conclude that mNGS is a useful diagnostic tool, but should only be performed selectively in addition to routine diagnostics of infectious diseases. The limited number of patients and the retrospective study design do not allow any further conclusions. [ABSTRACT FROM AUTHOR]
- Subjects :
- COMMUNICABLE disease diagnosis
COMMUNICABLE diseases
BLOOD
GENOMICS
BLOOD collection
POLYMERASE chain reaction
FISHER exact test
RETROSPECTIVE studies
DESCRIPTIVE statistics
STREPTOCOCCUS
QUANTITATIVE research
MANN Whitney U Test
CHI-squared test
LONGITUDINAL method
CELL culture
ANTI-infective agents
MEDICAL records
ACQUISITION of data
COMPARATIVE studies
DATA analysis software
SEQUENCE analysis
MEDICAL practice
MICROBIAL genetics
Subjects
Details
- Language :
- English
- ISSN :
- 03008126
- Volume :
- 52
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Infection
- Publication Type :
- Academic Journal
- Accession number :
- 178774673
- Full Text :
- https://doi.org/10.1007/s15010-024-02300-2