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What Are the Key Factors for the Detection of Peptides Using Mass Spectrometry on Boron-Doped Diamond Surfaces?

Authors :
Aguedo, Juvissan
Vojs, Marian
Vrška, Martin
Nemcovic, Marek
Pakanova, Zuzana
Dragounova, Katerina Aubrechtova
Romanyuk, Oleksandr
Kromka, Alexander
Varga, Marian
Hatala, Michal
Marton, Marian
Tkac, Jan
Source :
Nanomaterials (2079-4991); Aug2024, Vol. 14 Issue 15, p1241, 19p
Publication Year :
2024

Abstract

We investigated the use of boron-doped diamond (BDD) with different surface morphologies for the enhanced detection of nine different peptides by matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS). For the first time, we compared three different nanostructured BDD film morphologies (Continuous, Nanograss, and Nanotips) with differently terminated surfaces (-H, -O, and -F) to commercially available Ground Steel plates. All these surfaces were evaluated for their effectiveness in detecting the nine different peptides by MALDI-MS. Our results demonstrated that certain nanostructured BDD surfaces exhibited superior performance for the detection of especially hydrophobic peptides (e.g., bradykinin 1–7, substance P, and the renin substrate), with a limit of detection of down to 2.3 pM. Further investigation showed that hydrophobic peptides (e.g., bradykinin 1–7, substance P, and the renin substrate) were effectively detected on hydrogen-terminated BDD surfaces. On the other hand, the highly acidic negatively charged peptide adrenocorticotropic hormone fragment 18–39 was effectively identified on oxygen-/fluorine-terminated BDD surfaces. Furthermore, BDD surfaces reduced sodium adduct contamination significantly. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20794991
Volume :
14
Issue :
15
Database :
Complementary Index
Journal :
Nanomaterials (2079-4991)
Publication Type :
Academic Journal
Accession number :
178952033
Full Text :
https://doi.org/10.3390/nano14151241