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Down-regulation of immediate early gene egr-1 expression in rat C6 glioma cells by short-term exposure to high salt culture medium
- Source :
- Cell Biology International; Apr2005, Vol. 29 Issue 4, p261-268, 8p
- Publication Year :
- 2005
-
Abstract
- Abstract: Influence of high salt culture conditions on the expression of immediate early gene egr-1 in rat C6 glioma cells was investigated by measuring both Egr-1 mRNA and protein levels in the cells exposed to the medium containing high concentrations of NaCl. The exposure to high salt medium reduced Egr-1 mRNA and protein levels, while Egr-1 mRNA levels were not altered by the medium containing either sucrose or glycerol. Veratridine and monensin also reduced Egr-1 mRNA levels, similar in extent to that induced by high salt medium. Imaging analysis indicated that the exposure to high salt medium induced the elevation of Na<superscript>+</superscript> levels within the cells. These results indicate that neither hyperosmotic pressure nor ionic strength of high salt medium contribute to the reduction of Egr-1 expression, and suggest that the elevation of intracellular Na<superscript>+</superscript> concentration is closely associated with the down-regulation of egr-1 gene expression. [Copyright &y& Elsevier]
- Subjects :
- GENE expression
GENETIC regulation
MESSENGER RNA
SALT
Subjects
Details
- Language :
- English
- ISSN :
- 10656995
- Volume :
- 29
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Cell Biology International
- Publication Type :
- Academic Journal
- Accession number :
- 17920569
- Full Text :
- https://doi.org/10.1016/j.cellbi.2005.01.002