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METTL3-Regulated lncRNA SNHG7 Drives MNNG-Induced Epithelial–Mesenchymal Transition in Gastric Precancerous Lesions.
- Source :
- Toxics; Aug2024, Vol. 12 Issue 8, p573, 15p
- Publication Year :
- 2024
-
Abstract
- As a representative item of chemical carcinogen, MNNG is closely associated with the onset of gastric cancer (GC), where N6-methyladonosine (m6A) RNA methylation is recognized as a critical epigenetic event. In our previous study, we found that the m6A modification by methyltransferase METTL3 was up-regulated in MNNG-exposed malignant GES-1 cells (MC cells) compared to control cells in vitro, and long non-coding RNA SNHG7 as a downstream target of the METTL3. However, the functional role of METTL3 in mediating the SNHG7 axis in MNNG-induced GC remains unclear. In the present study, we continuously investigate the functional role of METTL3 in mediating the SNHG7 axis in MNNG-induced GC. RIP-PCR and m6A-IP-qPCR were used to examine the molecular mechanism underlying the METTL3/m6A/SNHG7 axis in MNNG-induced GC. A METTL3 knockout mice model was constructed and exposed by MNNG. Western blot analysis, IHC analysis, and RT-qPCR were used to measure the expression of METTL3, SNHG7, and EMT markers. In this study, we demonstrated that in MNNG-induced GC tumorigenesis, the m6A modification regulator METTL3 facilitates cellular EMT and biological functions through the m6A/SNHG7 axis using in vitro and in vivo models. In conclusion, our study provides novel insights into critical epigenetic molecular events vital to MNNG-induced gastric carcinogenesis. These findings suggest the potential therapeutic targets of METTL3 for GC treatment. [ABSTRACT FROM AUTHOR]
- Subjects :
- RNA methylation
LINCRNA
WESTERN immunoblotting
PRECANCEROUS conditions
KNOCKOUT mice
Subjects
Details
- Language :
- English
- ISSN :
- 23056304
- Volume :
- 12
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Toxics
- Publication Type :
- Academic Journal
- Accession number :
- 179380309
- Full Text :
- https://doi.org/10.3390/toxics12080573