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Preparation and characterization of a collagen/chitosan/heparin matrix for an implantable bioartificial liver.
- Source :
- Journal of Biomaterials Science -- Polymer Edition; Sep2005, Vol. 16 Issue 9, p1063-1080, 18p
- Publication Year :
- 2005
-
Abstract
- A new type of collagen/chitosan/heparin matrix, fabricated by gelation of collagen/chitosan with heparin sodium containing ammonia, was produced to construct livers by tissue engineering and regenerative engineering. The obtained collagen/chitosan/heparin matrix was found to be highly porous, swelled rapidly in PBS solution and was stable in vitro for at least 60 days in collagenase/lysozyme containing buffered aqueous solution (PBS, pH 7.4) at 37°C. The collagen/chitosan/heparin matrix resulted in a superior blood compatibility compared to the ammonia-treated collagen and collagen/chitosan matrices. The morphology and behavior of the cells on the collagen/chitosan/heparin membrane were found to be similar to those on the collagen membrane but different from those on the collagen/chitosan membrane. Hepatocytes cultured on the collagen/chitosan/heparin matrices exhibited highest urea and triglyceride secretion functions 25 days post seeding. These results suggest that this collagen/chitosan/heparin matrix is a potential candidate for liver tissue engineering. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09205063
- Volume :
- 16
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Journal of Biomaterials Science -- Polymer Edition
- Publication Type :
- Academic Journal
- Accession number :
- 17980702
- Full Text :
- https://doi.org/10.1163/1568562054798554