All that glitters is not gold: An interpretive framework for diagnostic test evaluation using Ascaris lumbricoides as a conceptual example.

The article discusses the challenges of evaluating diagnostic tests for neglected tropical diseases (NTDs) and proposes an interpretive framework using Ascaris lumbricoides as an example. The authors argue that using a single "gold standard" reference test can lead to biased estimates of sensitivity and specificity. They recommend using latent class models (LCMs) to analyze data from multiple imperfect tests and account for correlations between test results. The article emphasizes the importance of considering the purpose of testing and the target population when evaluating diagnostic tests for NTDs. The authors provide a graphical illustration to help researchers interpret the results and suggest that the framework should be considered before data collection to modify the combination of tests and populations if necessary. They also highlight the need for further guidance and standardization in diagnostic test evaluation for NTDs. [Extracted from the article]