Jiangsu University Reports Findings in Escherichia coli O157:H7 (Ultrasensitive Analysis of Escherichia coli O157:H7 Based on Immunomagnetic Separation and Labeled Surface-Enhanced Raman Scattering with Minimized False Positive Identifications).

A study conducted by Jiangsu University in China has developed an ultrasensitive method for detecting Escherichia coli O157:H7, a pathogen commonly found in food. The method combines immunomagnetic separation and labeled surface-enhanced Raman scattering (SERS) to achieve high selectivity in pathogen monitoring. The researchers used monoclonal antibodies and aptamers to identify and separate the bacteria, and a plasmonic nanostructure with embedded Raman tags for detection. The method showed good linearity and a limit of detection of 2 CFU/mL, making it a promising tool for on-site food pathogenic bacterial detection. The research has been peer-reviewed and published in the Journal of Agricultural and Food Chemistry. [Extracted from the article]