Nanoparticles in Managing Bovine Mastitis Pathogens—Isolation, Identification, and Therapeutic Strategies.

Nowadays, mastitis is one of the most common diseases in breeding dairy cattle. Treatment with conventional antibiotics is ineffective because many pathogens are resistant. This study aims to investigate the presence of mastitis in milk samples from cows exhibiting clinical symptoms, along with assessing the sensitivity of the bacteria isolated from these samples to various antibiotics. The research involved analysing 70 milk samples obtained from cows with clinical mastitis at the Veterinary Department, Banas Dairy, Palanpur. From these samples, a total of 12 different bacterial strains were identified. Identification methods included conventional biochemical techniques and MALDI-TOF analysis. Furthermore, the study evaluated the antibacterial properties of Copper oxide nanoparticles (CuO-NPs), Triphala nanoparticles, and Chitosan nanoparticles, the nanoparticles were characterized by SEM and FTIR. The mean size of chitosan, Copper and Triphala were 380 nm, 400 nm and 400 nm with zeta potential + 44.3 mv, -42.2 mv and -27.5 mv respectively. These nanoparticles were explored as potential alternatives for mastitis treatment in cows. The research also aimed to determine the minimum inhibitory concentration (MIC) of CuO-NPs, Triphala NPs, and Chitosan NPs against the bacterial strains isolated from the milk samples. By examining the efficacy of these nanoparticles and their MIC against mastitis-causing bacteria, this study contributes to the ongoing efforts to find effective treatments for mastitis in dairy cows. [ABSTRACT FROM AUTHOR]