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A H2O2 generation-detection-regulation integrated platform for boosting the efficiency of peroxygenase-catalysed C-H oxidative hydroxylation.
A H2O2 generation-detection-regulation integrated platform for boosting the efficiency of peroxygenase-catalysed C-H oxidative hydroxylation.
- Source :
- Green Synthesis & Catalysis; Aug2024, Vol. 5 Issue 3, p153-158, 6p
- Publication Year :
- 2024
-
Abstract
- The peroxygenases are ideal biocatalysts for the selective oxyfunctionalisation of stable C-H bonds. However, the catalytic efficiency of this approach is limited due to enzyme lability toward oxidant H<subscript>2</subscript>O<subscript>2</subscript>. Although the reported in-situ H<subscript>2</subscript>O<subscript>2</subscript> generation system enables the stable biocatalytic process without deactivating the enzyme, the greatest catalytic potential of peroxygenases still cannot be fulfilled effectively. To address the above issue, a H<subscript>2</subscript>O<subscript>2</subscript> generation-detection-regulation platform that integrated an effective organocatalyst-driven H<subscript>2</subscript>O<subscript>2</subscript> generation system, a precise electrochemical H<subscript>2</subscript>O<subscript>2</subscript> real-time detection device, and a convenient H<subscript>2</subscript>O<subscript>2</subscript> regulation strategy was first developed. The suitable range of H<subscript>2</subscript>O<subscript>2</subscript> generation rate for maximizing the catalytic efficiency of perox-ygenases while minimizing inactivation of the enzyme was firstly obtained by simply adjusting the amount of organocatalyst. According to the determined suitable range, the C-H oxyfunctionalisation efficiency of peroxygenases for each substrate was significantly boosted, achieving ~3-fold of the reported highest turnover frequency. [ABSTRACT FROM AUTHOR]
- Subjects :
- HYDROGEN peroxide
CARBON-hydrogen bonds
ENZYMES
ORGANOCATALYSIS
HYDROXYLATION
Subjects
Details
- Language :
- English
- ISSN :
- 26665549
- Volume :
- 5
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Green Synthesis & Catalysis
- Publication Type :
- Academic Journal
- Accession number :
- 180157454
- Full Text :
- https://doi.org/10.1016/j.gresc.2023.11.006