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A H2O2 generation-detection-regulation integrated platform for boosting the efficiency of peroxygenase-catalysed C-H oxidative hydroxylation.

A H2O2 generation-detection-regulation integrated platform for boosting the efficiency of peroxygenase-catalysed C-H oxidative hydroxylation.

Authors :
Xiaowang Zhang
Zhuotao Tan
Mengjiao Xu
Wei Zhuang
Hanjie Ying
Zhenyu Chu
Chenjie Zhu
Source :
Green Synthesis & Catalysis; Aug2024, Vol. 5 Issue 3, p153-158, 6p
Publication Year :
2024

Abstract

The peroxygenases are ideal biocatalysts for the selective oxyfunctionalisation of stable C-H bonds. However, the catalytic efficiency of this approach is limited due to enzyme lability toward oxidant H<subscript>2</subscript>O<subscript>2</subscript>. Although the reported in-situ H<subscript>2</subscript>O<subscript>2</subscript> generation system enables the stable biocatalytic process without deactivating the enzyme, the greatest catalytic potential of peroxygenases still cannot be fulfilled effectively. To address the above issue, a H<subscript>2</subscript>O<subscript>2</subscript> generation-detection-regulation platform that integrated an effective organocatalyst-driven H<subscript>2</subscript>O<subscript>2</subscript> generation system, a precise electrochemical H<subscript>2</subscript>O<subscript>2</subscript> real-time detection device, and a convenient H<subscript>2</subscript>O<subscript>2</subscript> regulation strategy was first developed. The suitable range of H<subscript>2</subscript>O<subscript>2</subscript> generation rate for maximizing the catalytic efficiency of perox-ygenases while minimizing inactivation of the enzyme was firstly obtained by simply adjusting the amount of organocatalyst. According to the determined suitable range, the C-H oxyfunctionalisation efficiency of peroxygenases for each substrate was significantly boosted, achieving ~3-fold of the reported highest turnover frequency. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
26665549
Volume :
5
Issue :
3
Database :
Complementary Index
Journal :
Green Synthesis & Catalysis
Publication Type :
Academic Journal
Accession number :
180157454
Full Text :
https://doi.org/10.1016/j.gresc.2023.11.006