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Differential expression of FSTL1 and its correlation with the pathological process of periodontitis.

Authors :
Jiang, Wenxin
Yu, Weijun
Hu, Shucheng
Shi, Yuanjie
Lin, Lu
Yang, Ruhan
Tang, Jiaqi
Gu, Yuting
Gong, Yuhua
Jin, Min
Lu, Eryi
Source :
Journal of Periodontal Research; Oct2024, Vol. 59 Issue 5, p1005-1016, 12p
Publication Year :
2024

Abstract

Aims: This study aimed to elucidate the alterations in Follistatin‐like protein 1 (FSTL1) and its association with the pathological process of periodontitis. Methods: This study included 48 patients with periodontitis and 42 healthy controls. The expression level of FSTL1 in the gingiva was determined by RT‐qPCR, validated using the dataset GSE16134, and subsequently examined by western blotting. Bioinformatics analysis revealed a single‐cell distribution of FSTL1, characteristic of angiogenesis and immune cell infiltration. The expression and distribution of FSTL1, vascular endothelial marker protein CD31 and myeloperoxidase (MPO), the indicator of neutrophil activity, were determined by immunohistochemistry (IHC). A series of correlation analyses was performed to determine the associations between FSTL1 and clinical parameters, including probing depth (PD) and clinical attachment loss (CAL), and their potential role in angiogenesis (CD31) and neutrophil infiltration (MPO). Results: FSTL1 was significantly upregulated in the gingiva of patients with periodontitis compared to their healthy counterparts. In addition, FSTL1 was positively correlated with the clinical parameters PD (r =.5971, p =.0005) and CAL (r =.6078, p =.0004). Bioinformatic analysis and IHC indicated that high FSTL1 expression was significantly correlated with angiogenesis and neutrophil infiltration in periodontitis. Moreover, receiver operating characteristic (ROC) analysis demonstrated that FSTL1 could serve as an independent indicator for evaluating the severity of periodontitis (area under the curve [AUC] = 0.9011, p <.0001). Conclusion: This study demonstrated FSTL1 upregulation in periodontitis and its potential contribution to the disease via angiogenesis and neutrophil infiltration. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00223484
Volume :
59
Issue :
5
Database :
Complementary Index
Journal :
Journal of Periodontal Research
Publication Type :
Academic Journal
Accession number :
180410505
Full Text :
https://doi.org/10.1111/jre.13275