Study on optimization of extraction process from Phellodendron amurense Rupr. polysaccharide by response surface methodology and its antioxidant activity.

The experiment used the response surface method to optimize the extraction process of polysaccharides from Phellodendron amurense Rupr. and studied its antioxidant activity. Four factors with significant impact on polysaccharide extraction (extraction temperature, extraction time, ultrasonic power, and liquid-to-solid ratio) were selected as the subjects of investigation. Based on the results of single-factor experiments, the Box-Behnken design response surface method was used to optimize the extraction process of Phellodendron amurense Rupr. polysaccharides. The antioxidant activity of Phellodendron amurense Rupr. polysaccharides was evaluated by measuring their scavenging effects on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radicals and hydroxyl radicals. The results showed that the optimal extraction process obtained by the response surface optimization was an extraction temperature of 79 °C, an extraction time of 54 minutes, an ultrasonic power of 59 W, and a liquid-to-solid ratio of 23 mL/g, at which the yield of Phellodendron amurense Rupr. reached 3.19%. At a concentration of 5 g/L, the maximum scavenging rates of DPPH and hydroxyl radicals by Phellodendron amurense Rupr. polysaccharides were 61% and 39%, respectively. The study indicates that the optimized ultrasonic extraction process of Phellodendron amurense Rupr. polysaccharides is stable and feasible, and Phellodendron amurense Rupr. polysaccharides possess certain antioxidant activity. [ABSTRACT FROM AUTHOR]