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Analysis of the Proliferative T Cell Response to Human Cytomegalovirus Major Immediate-Early Protein (IE1): Phenotype, Frequency and Variability.
- Source :
- Scandinavian Journal of Immunology; Mar1995, Vol. 41 Issue 3, p247-255, 9p, 1 Diagram, 2 Charts, 2 Graphs
- Publication Year :
- 1995
-
Abstract
- Cellular immune responses are important in the recovery from human cytomegalovirus (HCMV) infection. However, little is known about the CD4<superscript>+</superscript> T cell response and the target antigens (Ag) recognized. In this paper, we have analysed the proliferative T cell response of healthy HCMV seropositive (HCMV<superscript>+</superscript>) blood donors to recombinant immediate-early proteins expressed in transfected astrocytoma cells and to total HCMV Ags expressed in infected astrocytoma cells. We found that CD4<superscript>+</superscript> T cells were the major cell population that proliferated in the presence of IE or total HCMV Ags. Among healthy HCMV seropositive blood donors with anti-HCMV specific proliferative response, 33-44% also responded to IE Ags. Moreover, in high responders, the precursor frequencies of cells which proliferated in the presence of total HCMV, IE, or IE1 Ags were high (1/103 to 1/255, 1/2785 to 1/7744 and 1/5190 to 1/13531, respectively). In some donors, the anti-IE response was variable over time, whereas the anti-total HCMV Ags response remained constant, which suggests regulation of the anti-IE response in immunocompetent subjects. Our results suggest that the CD4<superscript>+</superscript> anti-IE1 response represents a significant part of the anti-HCMV proliferative response, both at the population level, and within individual immune systems. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03009475
- Volume :
- 41
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Scandinavian Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 18720465
- Full Text :
- https://doi.org/10.1111/j.1365-3083.1995.tb03560.x