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Immunobiological Studies on Experimental Visceral Leishmaniasis IV. Kinetics of Evolution of Disease-Promoting Versus Host-Protective Cells of Monocyte-Macrophage Lineage and their Characterization.
- Source :
- Scandinavian Journal of Immunology; Nov1995, Vol. 42 Issue 5, p540-546, 7p, 6 Graphs
- Publication Year :
- 1995
-
Abstract
- The evolution of cells of the monocyte-macrophage lineage (MML cells) in the spleen of <em>Leishmania donovani</em> (LD) infected BALB/c mice was studied. Spleen cells were fractionated on a discontinuous percoll gradient and adherent cells (AC) were purified from fractionated spleen cells by adherence steps that appeared at the interfaces of 25-35%, 35-40%, 40-45% and 45-50% percoll gradients. The AC were characterized as MML cells on the basis of positive staining for non-specific esterase. Adherent cells that appeared at the interfaces of 25-35% and 40-45% were defined as A and C, respectively, and both of them showed extreme variation in a progressive infection. It was observed that A supported parasite replication whereas C remained refractory when infected with LD <em>in vitro</em>. Furthermore, when A cells and C cells were used as antigen-presenting cells to stimulate mixed population of IFN-γ producing and IL-4 producing T-cells, it was observed that IL-4 and IFN-γ were the predominating cytokine in the T-cell supernatant, respectively. Both A and C were found to be increased hand-in-hand up to 5 months of infection and from then on A decreased and C increased in their numerical strength (A-C reciprocity). The evolution of A-C reciprocity coincided with the gradual reduction in the parasitaemia in the spleen suggesting that this may contribute to the acquisition of anti-leishmania immunity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03009475
- Volume :
- 42
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Scandinavian Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 18732412
- Full Text :
- https://doi.org/10.1111/j.1365-3083.1995.tb03693.x