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Bi-Enzyme Alcohol Biosensors Based on Genetically Engineered Alcohol Oxidase and Different Peroxidases.
- Source :
- Microchimica Acta; Dec2005, Vol. 152 Issue 1/2, p21-27, 7p
- Publication Year :
- 2005
-
Abstract
- We report on the development of a bi-layer bi-enzyme biosensor architecture using different peroxidases and alcohol oxidase from Hansenula polymorpha C-105 as biological recognition elements. The sensor architecture comprises a first layer containing either horseradish peroxidase or royal palm tree peroxidase crosslinked with an Osmium complex-modified redox hydrogel. On top, a second layer was formed by electrochemically induced precipitation of a cathodic electrodeposition paint simultaneously entrapping alcohol oxidase isolated from a genetically modified strain of Hansenula polymorpha C-105. The sensor architecture was optimized with respect to effective electron transfer and stability of the enzyme. The main characteristics of the biosensors are an apparent maximal current I<subscript>max</subscript> <superscript>app</superscript> of 572–940 nA, an apparent Michaelis constant K<subscript>M</subscript> <superscript>app</superscript> of 9.5 mM, a sensitivity of 60–98 nA mM<superscript>−1</superscript> and an improved operational stability represented by a deactivation constant of 1.5–2.0 × 10<superscript>−4</superscript>min<superscript>−1</superscript>. [ABSTRACT FROM AUTHOR]
- Subjects :
- BIOSENSORS
DETECTORS
ENZYMES
CHEMICAL detectors
ALCOHOL
OXIDASES
PEROXIDASE
Subjects
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 152
- Issue :
- 1/2
- Database :
- Complementary Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 18813298
- Full Text :
- https://doi.org/10.1007/s00604-005-0407-7