Back to Search
Start Over
A Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus dabanensis D-8T: cloning and molecular characterization.
- Source :
- FEMS Microbiology Letters; Feb2006, Vol. 255 Issue 1, p89-95, 7p, 2 Diagrams, 4 Graphs
- Publication Year :
- 2006
-
Abstract
- A gene encoding a Na<superscript>+</superscript>/H<superscript>+</superscript> antiporter was cloned from a chromosomal DNA of Halobacillus dabanensis strain D-8<superscript>T</superscript> by functional complementation. Its presence enabled the antiporter-deficient Escherichia coli strain KNabc to survive in the presence of 0.2 M NaCl or 5 mM LiCl. The gene was sequenced and designated as nhaH. The deduced amino-acid sequence of NhaH consists of 403 residues with a calculated molecular mass of 43 481 Da, which was 54% identical and 76% similar to the NhaG Na<superscript>+</superscript>/H<superscript>+</superscript> antiporter of Bacillus subtilis. The hydropathy profile was characteristic of a membrane protein with 12 putative transmembrane domains. Everted membrane vesicles prepared from E. coli cells carrying nhaH exhibited Na<superscript>+</superscript>/H<superscript>+</superscript> as well as Li<superscript>+</superscript>/H<superscript>+</superscript> antiporter activity, which was pH-dependent with highest activities at pH 8.5–9.0 and at pH 8.5, respectively. Moreover, nhaH confers upon E. coli KNabc cells the ability to grow under alkaline conditions. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03781097
- Volume :
- 255
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- FEMS Microbiology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 20005439
- Full Text :
- https://doi.org/10.1111/j.1574-6968.2005.00055.x