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Silencing of filamin A gene expression inhibits Ca2+-sensing receptor signaling
- Source :
- FEBS Letters; Mar2006, Vol. 580 Issue 7, p1795-1800, 6p
- Publication Year :
- 2006
-
Abstract
- Abstract: Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca<superscript>2+</superscript>-sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function. [Copyright &y& Elsevier]
- Subjects :
- GENE expression
ACTIN
CYTOSKELETON
PROTEINS
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 580
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- FEBS Letters
- Publication Type :
- Academic Journal
- Accession number :
- 20186441
- Full Text :
- https://doi.org/10.1016/j.febslet.2006.02.035