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Binding of heterologous anti-brain antibodies to mouse B cells.
- Source :
- Immunology; Jun76, Vol. 30 Issue 6, p859-864, 6p, 2 Black and White Photographs, 1 Chart, 2 Graphs
- Publication Year :
- 1976
-
Abstract
- Antibodies produced in rabbits against mouse brain are able to react with the majority of lymph node and spleen murine lymphocytes, as demonstrated by indirect immunofluorescence. Cells stained by a suitably absorbed rabbit anti-mouse brain serum showed various degrees of fluorescence, from bright fluorescent rings to a few speckles per cell; lymphocytes from the spleen of deprived mice (B mice) predominantly stained in small caps. Double labelling experiments in which B cells were identified by their ‘easily detectable’ surface immunoglobulin revealed that a great proportion of these cells had two overlapping caps, a large one formed by the anti-immunoglobulin serum and a small one formed by the anti-brain serum. Up to 10 per cent of these spleen B cells, however, appeared as strongly fluorescent as T cells, with the suitably absorbed heterologous anti-brain serum. No anti-immunoglobulin activity was detected in this serum. It is therefore suggested that some B cells may bear on their surfaces a θ or θ-related antigen similar to that on T cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- IMMUNOGLOBULINS
LYMPH nodes
LYMPHOCYTES
IMMUNOFLUORESCENCE
SERUM
ANTIGENS
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 30
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 20442415