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The Ina and Inb blood group antigens are located on a glycoprotein of 80,000 MW (the CDw44 glycoprotein) whose expression is influenced by the In(Lu) gene.
- Source :
- Immunology; May88, Vol. 64 Issue 1, p37-43, 7p, 2 Diagrams, 1 Chart, 1 Graph
- Publication Year :
- 1988
-
Abstract
- The In<superscript>a</superscript> and In<superscript>b</superscript> blood group antigens were found to he located on an erythrocyte membrane glycoprotein of 80,000 MW by immunoblotting with human anti-In<superscript>a</superscript> and anti-In<superscript>b</superscript> antibodies under non-reducing conditions. This glycoprotein is shown here to be identical to that defined by monoclonal antibodies to CDw44, and a new murine monoclonal antibody (BRIC 35) is added to this cluster. Experiments with endo-β-galactosidase and Endo F preparations suggest that the glycoprotein contains one or more N-glycans but that these oligosaccharides do not contain extensive poly-N-acetyl lacto saminyl sequences. Experiments using membranes prepared from sialidase-treated normal erythrocytes, from Tn erythrocytes and from Cad erythrocytes suggest that the glycoprotein does not contain a substantial content of O-glycans, The In<superscript>b</superscript> antigen and the epitope defined by a murine monoclonal antibody (BRIC 35) show reduced expression on Lu(a-b-) erythrocytes which result from the effect of the dominant inhibitor gene In(Lu). Evidence is presented here that the In<superscript>b</superscript> antigen is expressed on normal granulocytes and lymphocytes and on the haemopoietic cell lines HEL, K562 and HL-60, a lymphoblastoid cell line and lymphocytes from two patients with B-CLL. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANTIGENS
ERYTHROCYTES
GLYCOPROTEINS
MONOCLONAL antibodies
LYMPHOCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 64
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 20894563