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rpoB sequence analysis as a novel basis for bacterial identification.
- Source :
- Molecular Microbiology; Dec1997, Vol. 26 Issue 5, p1005-1011, 7p, 2 Diagrams, 2 Charts, 1 Graph
- Publication Year :
- 1997
-
Abstract
- Comparison of the sequences of conserved genes, most commonly those encoding 16S rRNA, is used for bacterial genotypic identification. Among some taxa, such as the Enterobacteriaceae, variation within this gene does not aiiow confident species identification. We investigated the usefulness of RNA polymerase beta-subunit encoding gene (<em>rpoB</em>) sequences as an alternative tool for universal bacterial genotypic identification. We generated a database of partial <em>rpoB</em> for 14 Enterobacteriaceae species and then assessed the intra- and interspecies divergence between the <em>rpoB</em> and the 16S rRNA genes by pairwise comparisons. We found that levels of divergence between the <em>rpoB</em> sequences of different strains were markedly higher than those between their 16S rRNA genes. This higher discriminatory power was further confirmed by assigning 20 blindly selected clinical isolates to the correct enteric species on the basis of <em>rpoB</em> sequence comparison. Comparison of <em>rpoB</em> sequences from Entero-bacteriaceae was also used as the basis for their phylogenetic analysis and demonstrated the genus <em>Klebsiella</em> to be polyphyletic. The trees obtained with <em>rpoB</em> were more compatible with the currently accepted classification of Enterobacteriaceae than those obtained with 16S rRNA. These data indicate that <em>rpoB</em> is a powerful identification tool, which may be useful for universal bacterial identification. [ABSTRACT FROM AUTHOR]
- Subjects :
- ENTEROBACTERIACEAE
RNA polymerases
NUCLEOTIDE sequence
BACTERIA
GENES
PHYLOGENY
Subjects
Details
- Language :
- English
- ISSN :
- 0950382X
- Volume :
- 26
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Molecular Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 21323388
- Full Text :
- https://doi.org/10.1046/j.1365-2958.1997.6382009.x