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Studies on the overexpression of the soybean GmNHX1 in Lotus corniculatus: The reduced Na+ level is the basis of the increased salt tolerance.
- Source :
- Chinese Science Bulletin; Jun2006, Vol. 51 Issue 11, p1306-1315, 10p, 5 Diagrams, 5 Graphs
- Publication Year :
- 2006
-
Abstract
- Abstract The full length cDNA coding for a novel vacuolar Na<superscript>+</superscript>/H<superscript>+</superscript> antiporter (GmNHX1) was cloned from soybean and determined to consist of 2591 bp with a 5′-untranslated region of 464 bp, an open reading frame (ORF) of 1641 bp, and a 3′-untranslated region of 466 bp. The deduced protein sequence contains 546 aa with the typical characters of the vacuolar Na<superscript>+</superscript>/H<superscript>+</superscript> antiporters, and shares high similarity with that of AtNHX1, OsNHX1 and AgNHX1. The soybean genome showed a single copy of the GmNHX1 gene. Northern blot analysis demonstrated that the expression of the GmNHX1 was tissue-specific, increased by ABA treatment, NaCl, KCl, LiCl and dehydration stress, and lower in leaves but higher in roots and hypocotyls of salt-tolerant than salt-sensitive cultivars. The GmNHX1 was overexpressed under the control of a tandem cauliflower mosaic virus (CaMV) 35S promoter in the model leguminous plant Lotus corniculatus L. and conferred salt-tolerance of the transgenic plants. Measurements of Na<superscript>+</superscript> and K<superscript>+</superscript> contents in both roots and shoots demonstrated that the plantlets of GmNHX1-overexpressing lines had lower Na<superscript>+</superscript> and K<superscript>+</superscript> content, and higher K<superscript>+</superscript>/Na<superscript>+</superscript> ratio than the controlled lines that were transformed with the empty vector, which indicates that the salt-tolerance conferred by GmNHX1 is closely related with decreased accumulation of Na<superscript>+</superscript> in the transgenic plants. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10016538
- Volume :
- 51
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Chinese Science Bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 22186677
- Full Text :
- https://doi.org/10.1007/s11434-006-1306-y