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Interaction of Bacillus thuringiensis svar. israelensis Cry toxins with binding sites from Aedes aegypti (Diptera: Culicidae) larvae midgut.

Authors :
de Barros Moreira Beltrão, Henrique
Silva-Filha, Maria Helena Neves Lobo
Source :
FEMS Microbiology Letters; Jan2007, Vol. 266 Issue 2, p163-169, 7p, 2 Charts, 2 Graphs
Publication Year :
2007

Abstract

This work shows in vitro processing of Bacillus thuringiensis svar. isralensis Cry toxins and the capacity of the active fragments to bind the midgut microvilli of Aedes aegypti larvae. Processing of Cry11Aa, Cry4Aa and Cry4Ba yielded double fragments of 38–30, 45–20 and 45–18 kDa, respectively. Competition assays showed that all active <superscript>125</superscript>I-Cry toxins are able to specifically bind to brush border membrane fractions and they might share a common class of binding sites. The values of IC<subscript>50</subscript> suggested that toxins do not display high affinity for the receptors from brush border membrane fractions, while dissociation assays showed that binding was irreversible, indicating the insertion of toxins in the cell membrane. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03781097
Volume :
266
Issue :
2
Database :
Complementary Index
Journal :
FEMS Microbiology Letters
Publication Type :
Academic Journal
Accession number :
23415148
Full Text :
https://doi.org/10.1111/j.1574-6968.2006.00527.x