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Effect of Antiestrogens on EGF-Mediated Movement of Human Breast Cancer Cells.
- Source :
- Pharmacology; 2007, Vol. 79 Issue 2, p93-96, 4p, 2 Graphs
- Publication Year :
- 2007
-
Abstract
- In a previous study we compared the influence of several growth factors on breast cancer cells in culture and observed that epidermal growth factor (EGF) enhanced the invasiveness of estrogen receptor-positive breast cancer cells. The objective of the present study was to determine the influence of three unique antiestrogens on EGF-mediated movement of human breast cancer cells. The rate of movement of MCF-7 breast cancer cells was measured using time-lapse videomicroscopy (TLVM). The MCF-7 cells were pretreated with antiestrogen (either tamoxifen, ICI-182-780 (ICI) or 1,1-dichloro-cis-2,3-diarylcyclopropane (AII)) at 10<superscript>–6</superscript> mol/l for 4 days, and then treated with EGF (10<superscript>–10</superscript> mol/l) immediately prior to TLVM. EGF enhanced the motility of the MCF-7 cells at 30–90 min post-administration. However, EGF-mediated motility of the MCF-7 cells was inhibited by antiestrogen pretreatment, with TAM and ICI producing complete inhibition of EGF-induced motility. In conclusion, this study demonstrates that EGF enhances the rate of movement of MCF-7 breast cancer cells and that antiestrogen pretreatment inhibits EGF-mediated motility. Copyright © 2007 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Subjects :
- ESTROGEN antagonists
BREAST cancer
CANCER cells
EPIDERMAL growth factor
THERAPEUTICS
Subjects
Details
- Language :
- English
- ISSN :
- 00317012
- Volume :
- 79
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 24580938
- Full Text :
- https://doi.org/10.1159/000097867