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Structural and functional characterization of a novel, host penetration-related pectate lyase from the potato cyst nematode Globodera rostochiensis.
- Source :
- Molecular Plant Pathology; May2007, Vol. 8 Issue 3, p293-305, 13p, 1 Color Photograph, 2 Black and White Photographs, 3 Diagrams, 1 Chart, 1 Graph
- Publication Year :
- 2007
-
Abstract
- The cell wall, a strong extraprotoplasmic layer surrounding plant cells that mainly consists of a variety of polysaccharides, constitutes a major barrier for potential parasites. Plant-parasitic nematodes are well equipped to overcome this barrier as they produce and secrete cell-wall-degrading enzymes. Expression profiling of various life stages of the potato cyst nematode Globodera rostochiensis revealed a novel pectate lyase gene ( Gr-pel2, 759 bp). The Gr-PEL2 protein showed highest similarity to pectate lyases from the facultative plant-parasitic nematodes Bursaphelenchus mucronatus and B. xylophilus and the soil-inhabiting saprophytic Streptomyces and Frankia species (i.e. 40–42% identity and 58–60% similarity), whereas only a remote relatedness to the previously identified Gr-PEL1 was observed (i.e. 28% identity and 43% similarity). Transient expression of Gr-pel2 in leaves of Nicotiana benthamiana resulted in severe malformations of the infiltrated tissues, not relating to maceration and soft rot symptoms. Ca<superscript>2+</superscript> is known to be essential for pectate lyase activity, and the most likely calcium-binding site was identified in the Gr-PEL2 protein by combining homology modelling of the three-dimensional structure, site-directed mutagenesis and transient expression in leaves. A highly charged cleft in Gr-PEL2, which is likely to be involved in substrate binding and which is also significantly more hydrophobic in Gr-PEL1, was shown to be essential for protein activity. Our results underline the broad spectrum of pectate lyases and cell-wall-degrading enzymes necessary for successful parasitism by cyst nematodes. [ABSTRACT FROM AUTHOR]
- Subjects :
- GOLDEN nematode
PLANT cell walls
POLYSACCHARIDES
NEMATODES
POTATOES
BIOPOLYMERS
Subjects
Details
- Language :
- English
- ISSN :
- 14646722
- Volume :
- 8
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Molecular Plant Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 24719184
- Full Text :
- https://doi.org/10.1111/j.1364-3703.2007.00394.x