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A new role of Deinococcus radiodurans RecD in antioxidant pathway.

Authors :
Qing Zhou
Xinjue Zhang
Hong Xu
Bujin Xu
Yuejin Hua
Source :
FEMS Microbiology Letters; Jun2007, Vol. 271 Issue 1, p118-125, 8p
Publication Year :
2007

Abstract

In Deinococcus radiodurans, RecBCD holoenzyme is not intact because of the absence of RecB and RecC, but a RecD-like protein does indeed exist. In this work, D. radiodurans recD disruptant was constructed and its possible biological functions were investigated. The results showed that disruption of the recD gene of D. radiodurans resulted in a remarkably increased sensitivity to hydrogen peroxide but had no apparent effect on the resistance to γ and UV radiation. Furthermore, complementation experiments showed that Escherichia coli RecD, helicase domain or N-terminal domain of D. radiodurans RecD could not individually restore the resistant phenotype to hydrogen peroxide of the recD disruptant, whereas the complete D. radiodurans RecD protein could. Further studies showed that D. radiodurans RecD took part in antioxidant process by stimulating catalase activity and reactive oxygen species scavenging activity in D. radiodurans. These results suggest that D. radiodurans RecD has a new role in the antioxidant pathway. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03781097
Volume :
271
Issue :
1
Database :
Complementary Index
Journal :
FEMS Microbiology Letters
Publication Type :
Academic Journal
Accession number :
24977038
Full Text :
https://doi.org/10.1111/j.1574-6968.2007.00703.x