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Distribution of t(14;18)-positive, putative lymphoma precursor cells among B-cell subsets in healthy individuals.
- Source :
- British Journal of Haematology; Aug2007, Vol. 138 Issue 3, p349-353, 5p, 1 Chart, 2 Graphs
- Publication Year :
- 2007
-
Abstract
- The t(14;18)(q32;q21) is the characteristic chromosomal translocation of follicular lymphoma (FL). Highly sensitive polymerase chain reaction (PCR) techniques can also detect t(14;18)-sequences in the blood and lymphoid tissues of healthy individuals (HI). The aim of this study was to determine the immunophenotypic markers of t(14;18)-positive cells in HI and to relate these features to lymphocyte maturation. B cells from 10 subjects with t(14;18)-positive and three subjects with t(14;18)-negative peripheral blood mononuclear cells (PBMC) were fluorescence-activated cell sorted for antigen-naïve (CD27<superscript>−</superscript>), immunoglobulin M (IgM) memory (IgM<superscript>+</superscript>CD27<superscript>+</superscript>) and switched memory (IgM<superscript>−</superscript> CD27<superscript>+</superscript>) cells. t(14;18)-recombinations were detected by quantitative PCR. Among PBMC-positive subjects, t(14;18)-frequency was significantly higher in IgM memory (median: 380/10<superscript>6</superscript>) than in antigen-naïve (median: 16/10<superscript>6</superscript>) or switched memory (median: 5/10<superscript>6</superscript>) B cells. All PBMC-negative subjects nevertheless had detectable t(14;18) in sorted B cells; levels were lower than in PBMC-positive subjects, but had the same relative predominance. These results suggest that t(14;18) is generated during early B-cell development in the bone marrow and that affected cells may mature and expand in germinal centres. t(14;18)-frequency was highest in IgM memory cells, a B-cell subset that shares immunophenotypic similarities with FL. The significance of these cells as lymphoma precursors or indicators of lymphoma risk remains to be established. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00071048
- Volume :
- 138
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- British Journal of Haematology
- Publication Type :
- Academic Journal
- Accession number :
- 25617434
- Full Text :
- https://doi.org/10.1111/j.1365-2141.2007.06671.x