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Naturally acquired antibodies to polymorphic and conserved epitopes of Plasmodium falciparum merozoite surface protein 3.
- Source :
- Parasite Immunology; Aug2007, Vol. 29 Issue 8, p387-394, 8p, 1 Chart, 4 Graphs
- Publication Year :
- 2007
-
Abstract
- Many studies on the role of merozoite surface protein 3 (MSP3) in immunity against malaria have focused on a conserved section of MSP3. New evidence suggests that polymorphic sequences within MSP3 are under immune selection. We report a detailed analysis of naturally-acquired antibodies to allele-specific and conserved parts of MSP3 in a Kenyan cohort. Indirect and competition ELISA to heterologous recombinant MSP3 proteins were used for antibody assays, and parasites were genotyped for msp3 alleles. Antibody reactivity to allele-specific and conserved epitopes of MSP3 was heterogenous between individuals. Overall, the prevalence of allele-specific antibody reactivity was significantly higher (3D7-specific 54%, K1-specific 41%) than that to a recombinant protein representing a conserved portion of C-terminal MSP3 (24%, P <  0·01). The most abundant IgG subclass was IgG3, followed by IgG1. Allele-specific reactivity to the K1-type of MSP3 was associated with a lower risk of clinical malaria episodes during a 6-month follow-up in individuals who were parasitized at the start of the malaria transmission season (Relative risk 0·41 with 95% confidence interval 0·20–0·81, P =  0·011). The potential importance of allele-specific immunity to MSP3 should be considered in addition to immunity to conserved epitopes, in the development of an MSP3 malaria vaccine. [ABSTRACT FROM AUTHOR]
- Subjects :
- PLASMODIUM falciparum
PLASMODIUM
PROTEINS
BIOMOLECULES
PROTEOMICS
Subjects
Details
- Language :
- English
- ISSN :
- 01419838
- Volume :
- 29
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Parasite Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 25787758
- Full Text :
- https://doi.org/10.1111/j.1365-3024.2007.00951.x