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Generic surface modification strategy for sensing applications based on Au/SiO2 nanostructures.
- Source :
- Biointerphases; Jun2007, Vol. 2 Issue 1, p49-55, 7p, 1 Black and White Photograph, 2 Diagrams, 1 Chart, 1 Graph
- Publication Year :
- 2007
-
Abstract
- A generic protocol for the creation of material-mediated self-assembled patterns of streptavidin, defined solely by patterns of gold and SiO<subscript>2</subscript>, is presented. Protein-adsorption resistance of selected regions was obtained by material-specific adsorption of thiol-modified poly(ethylene)glycol (thiol-PEG) on gold followed by adsorption of poly-L-lysine (PLL) modified PEG (PLL-g-PEG) on SiO<subscript>2</subscript>. Selective streptavidin binding to either gold or SiO<subscript>2</subscript> (or both) was ensured by introducing biotin-modified thiolated (thiol-biotin) and/or biotin-modified PLL-g-PEG (PLL-g-PEGbiotin) compounds. The introduction of biotin did not influence the protein-adsorption resistance. On the macroscopic scale, the protein-adsorption-resistant properties and the streptavidin-binding capacity were optimized using quartz crystal microbalance with dissipation monitoring. The reproduction of micrometer-scale gold patterns on SiO<subscript>2</subscript> into patterns of streptavidin was verified using fluorescence microscopy, while the compatibility of the material-specific surface-modification strategy with nanoscale features was accomplished by modifying a localized surface plasmon resonance (LSPR) active template, defined by randomly distributed nanoapertures in a thin gold film on SiO<subscript>2</subscript>. The demonstrated compatibility of the latter substrate with LSPR-based label-free sensing of biorecognition reactions, combined with the fact that all compounds utilized are commercially available, makes the surface-modification protocol attractive as a generic surface modification solution for a broad range of biorecognition-based assays. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19348630
- Volume :
- 2
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Biointerphases
- Publication Type :
- Academic Journal
- Accession number :
- 26222377
- Full Text :
- https://doi.org/10.1116/1.2717926