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Polypeptide from Chlamys farreri attenuates murine thymocytes damage induced by ultraviolet B.
- Source :
- Acta Pharmacologica Sinica; Oct2007, Vol. 28 Issue 10, p1665-1670, 6p, 1 Chart, 4 Graphs
- Publication Year :
- 2007
-
Abstract
- Aim: Polypeptide from Chlamys farreri (PCF, molecular mass is 879) is a new marine polypeptide compound isolated from Chlamys farreri. This study investigates the possible protective roles and the mechanism of PCF against ultraviolet B (UVB)-induced apoptosis in murine thymocytes. Methods: The rate of apoptosis and caspase-3 activation was measured by flow cytometry. The expression of stress-response genes c-fos and c-jun was observed by RT—PCR. Western blot analysis was performed to determine the release of cytochrome c. Results: It was found that UVB induced murine thymocyte death. The cells treated with UVB showed an increase in cytochrome c release, caspase-3 activity, as well as in the expression of c-fos and c-jun. In addition, all were involved in UVB-induced cell apoptosis. Conclusion: Our present observations pointed to the ability of PCF to avert UVB-induced apoptosis in thymocytes by modulating c-fos and c-jun expression, cytochrome c release, and the consequent activation of caspase-3, which were essential components of the UV-induced cell apoptotic pathway. The results suggested that PCF is a promising protective substance against UV radiation. [ABSTRACT FROM AUTHOR]
- Subjects :
- CHLAMYS
GROWTH factors
PEPTIDES
APOPTOSIS
CELL death
Subjects
Details
- Language :
- English
- ISSN :
- 16714083
- Volume :
- 28
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Acta Pharmacologica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 26771343
- Full Text :
- https://doi.org/10.1111/j.1745-7254.2007.00621.x