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A Leishmania infantum cytosolic tryparedoxin activates B cells to secrete interleukin-10 and specific immunoglobulin.
- Source :
- Immunology; Apr2008, Vol. 123 Issue 4, p555-565, 11p, 3 Charts, 4 Graphs
- Publication Year :
- 2008
-
Abstract
- The immune evasion mechanisms of pathogenic trypanosomatids involve a multitude of phenomena such as the polyclonal activation of lymphocytes, cytokine modulation and the enhanced detoxification of oxygen reactive species. A trypanothione cascade seems to be involved in the detoxification process. It was recently described and characterized a tryparedoxin ( LiTXN1) involved in Leishmania infantum cytoplasmatic hydroperoxide metabolism. LiTXN1 is a secreted protein that is up-regulated in the infectious form of the parasite, suggesting that it may play an important role during infection. In the present study, we investigated whether recombinant LiTXN1 (r LiTXN1) affects T- and B-cell functions in a murine model. We observed a significant increase in the CD69 surface marker on the B-cell population in total spleen cells and on isolated B cells from BALB/c mice after in vitro rLiTXN1 stimulus. Activated B-cells underwent further proliferation, as indicated by increased [<superscript>3</superscript>H]thymidine incorporation. Cytokine quantification showed a dose-dependent up-regulation of interleukin (IL)-10 secretion. B cells were identified as a source of this secretion. Furthermore, intraperitoneal injection of r LiTXN1 into BALB/c mice triggered the production of elevated levels of r LiTXN1-specific antibodies, predominantly of the immunoglobulin M (IgM), IgG1 and IgG3 isotypes, with a minimum reactivity against other heterologous antigens. Taken together, our data suggest that r LiTXN1 may participate in immunopathological processes by targeting B-cell effector functions, leading to IL-10 secretion and production of specific antibodies. [ABSTRACT FROM AUTHOR]
- Subjects :
- IMMUNOGLOBULINS
LYMPHOCYTES
BIOLOGICAL transport
INTERLEUKIN-10
CELLULAR immunity
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 123
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 29985088
- Full Text :
- https://doi.org/10.1111/j.1365-2567.2007.02725.x