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Gambogic acid induces death inducer-obliterator 1-mediated apoptosis in Jurkat T cells.
- Source :
- Acta Pharmacologica Sinica; Mar2008, Vol. 29 Issue 3, p349-354, 6p, 2 Diagrams, 3 Graphs
- Publication Year :
- 2008
-
Abstract
- Aim: To explore the anticancer effects and the molecular mechanisms of gambogic acid (GA) on Jurkat cells. Methods: Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Annexin V-fluorescein-isothiocyanate/propidium iodide, DNA defragmentation, and comet assay were used to detect apoptosis. Western blotting was used to study the expression of death inducer-obliterator-1 (DIO-1), Bcl-2, NF-κB, and procaspase 3, as well as 2 activated subunits: p17 and p20. The subcellular localization of DIO-1 was examined by immunofluorescence and Hoechst33258 staining. Results: GA inhibited the proliferation of Jurkat cells with 50% inhibitory concentration values of 1.51±0.09 (24 h), 0.98±0.13 (48 h), and 0.67±0.12 μmol/L (72 h). GA was able to induce apoptosis of Jurkat cells. Treated by GA, the expression of DIO-1 was upregulated, and that of Bcl-2 and NF-κB was downregulated, leading to the activation of pro-caspase 3. GA induced the translocation of DIO-1 to the nucleus. Conclusion: GA suppressed the proliferation of Jurkat cells by apoptosis induction. DIO-1 triggered early-stage cell death in GA-treated Jurkat cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16714083
- Volume :
- 29
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Acta Pharmacologica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 30068626
- Full Text :
- https://doi.org/10.1111/j.1745-7254.2008.00762.x