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Structure and characterization of the gene cluster of the O-antigen of Escherichia coli O49 containing 4,6-dideoxy-4-[( S-3-hydroxybutanoylamino]-D-glucose.
- Source :
- Biochemistry (00062979); Apr2008, Vol. 73 Issue 4, p406-410, 5p, 2 Charts, 1 Graph
- Publication Year :
- 2008
-
Abstract
- An O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of enteropathogenic Escherichia coli O49 and studied by sugar analysis along with one- and two-dimensional <superscript>1</superscript>H- and <superscript>13</superscript>C-NMR spectroscopy. The following structure of the linear tetrasaccharide repeating unit of the O-polysaccharide was established: where d-Qui4N( S3HOBut) stands for 4,6-dideoxy-4-[( S)-3-hydroxybutanoylamino]- d-glucose and O-acetylation of GlcNAc is partial (∼30%). To our knowledge, no N-(3-hydroxybutanoyl) derivative of Qui4N has been hitherto found in bacterial polysaccharides. Gene functions of the O-antigen gene cluster of E. coli O49 were assigned by bioinformatics analysis and found to correspond to the O-polysaccharide structure. Two new genes were revealed and suggested to be responsible for synthesis and transfer of the 3-hydroxybutanoyl group. [ABSTRACT FROM AUTHOR]
- Subjects :
- ENDOTOXINS
POLYSACCHARIDES
ESCHERICHIA coli
ESCHERICHIA
ACETYLATION
Subjects
Details
- Language :
- English
- ISSN :
- 00062979
- Volume :
- 73
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Biochemistry (00062979)
- Publication Type :
- Academic Journal
- Accession number :
- 31892332
- Full Text :
- https://doi.org/10.1134/S0006297908040044