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Isolation, Culture and Characterization of Endothelial Cells from Human Hypertrophic Scar.
- Source :
- Endothelium; May/Jun2008, Vol. 15 Issue 3, p113-119, 7p, 6 Black and White Photographs, 1 Diagram
- Publication Year :
- 2008
-
Abstract
- Although the etiology underlying scar formation is not well understood, previous studies revealed that endothelial cells play a role in the pathogenesis of scar development. Recently, the authors developed a reliable technique to obtain endothelial cells from hypertrophic scars that involved separation of cells from the scar tissue matrix and isolation from other cell types. Using phase-contract and electron microscopy, the cells were observed to have a characteristic morphology consistent with cells of endothelial origin. The cells were further characterized as endothelial cells by assessment of endothelin (ET)-1 and intercellular adhesion molecule (ICAM) mRNA expression, and the presence of factor VIII antigen, CD34, CD31, and VE-cadherin. This isolation method provides a simple method for culturing endothelial cells from hypertrophic scar tissue and should prove useful for studying the role of endothelial cell involvement in scar formation. [ABSTRACT FROM AUTHOR]
- Subjects :
- ENDOTHELIUM
SCARS
ELECTRON microscopy
CELL adhesion molecules
MESSENGER RNA
Subjects
Details
- Language :
- English
- ISSN :
- 10623329
- Volume :
- 15
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Endothelium
- Publication Type :
- Academic Journal
- Accession number :
- 32707360
- Full Text :
- https://doi.org/10.1080/10623320802125169