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Antiapoptosis and Mitochondrial Effect of Pioglitazone Preconditioning in the Ischemic/reperfused Heart of Rat.
- Source :
- Cardiovascular Drugs & Therapy; Aug2008, Vol. 22 Issue 4, p283-291, 9p
- Publication Year :
- 2008
-
Abstract
- Abstract Purpose  Pioglitazone, used clinically in the treatment of type 2 diabetes mellitus, has been implicated as a regulator of cellular inflammatory and ischemic responses. The present study examined whether pioglitazone could inhibit cadiocyte apoptosis and reduce mitochondrial ultrastructure injury and membrane potential loss in the ischemic/reperfused heart of the rat. Furthermore, we investigated whether the protective effect of pioglitazone was related to opening of the mitochondrialATP-sensitive potassium channels. Methods  Adult male SpragueâDawley rats were subjected to 30 min of ischemia followed by 4 h of reperfusion. At 24 h before ischemia, rats were randomized to receive 0.9% saline, 5-hydroxydecanoate (5-HD, 10 mg kgâ1, i.v.) plus pioglitazone (3 mg kgâ1, i.v.) or pioglitazone (3 mg kgâ1, i.v.). One group served as sham control. We investigated mitochondrial structure, apoptosis rate and Bcl-2, Bax and Caspase-3 proteins by immunohistochemistry staining. RT-PCR was used to determine the expression of P38MAPKmRNA and JNKmRNA. Western blotting was used to measure the expression of P38MAPK, JNK and NFκB P65. A second group of rats were randomly divided into sham-operated, ischemia/reperfusion (I/R), pioglitazone treatment, 5-HDââpioglitazone and 5-HD groups and the size of myocardial infarction was determined. Primary cultured cardiomyocytes of neonatal SpragueâDawley rats were divided into control, hypoxia reoxygenation, different concentrations of pioglitazone and 5-HDââpioglitazone groups. JC-1 staining flowcytometry was used to examine mitochondrial membrane potential (ÎΨm). Results  Pioglitazone decreased mitochondrial ultrastructural damage compared to I/R, and reduced infarct size from 34.93â±â5.55% (I/R) to 20.24â±â3.93% (PâPâPâPâPâ Conclusion  Pioglitazone inhibited cadiocyte apoptosis and reduced mitochondrial ultrastructure injury and membrane potential loss in the ischemic/reperfused heart of rat. These protective effects of pioglitazone may be related to opening mitochondrialATP-sensitive potassium channels. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09203206
- Volume :
- 22
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Cardiovascular Drugs & Therapy
- Publication Type :
- Academic Journal
- Accession number :
- 33328045
- Full Text :
- https://doi.org/10.1007/s10557-008-6115-x