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Characterization of the Glycine Betaine Biosynthetic Genes in the Moderately Halophilic Bacterium Halobacillus dabanensis D-8T.
- Source :
- Current Microbiology; Oct2008, Vol. 57 Issue 4, p306-311, 6p, 1 Black and White Photograph, 3 Diagrams, 2 Graphs
- Publication Year :
- 2008
-
Abstract
- The gene cluster involved in the choline-glycine betaine conversion pathway was cloned from chromosomal DNA of the Gram-positive moderate halophile Halobacillus dabanensis D-8<superscript>T</superscript>. Nucleotide sequence analysis revealed four genes, designated gbsT, gbsI, gbsA, and gbsB, which are clustered in a 5.1-kb fragment. After heterologous expression of gbsAB in the Escherichia coli mutant strain PD141, the transformed cells were able to grow in a selective M63 medium containing 0.7 M NaCl and 1 mM choline, in contrast to the mutant strain. Glycine betaine biosynthesis was restored and its accumulation was confirmed by using <superscript>13</superscript>C nuclear magnetic resonance spectroscopy. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03438651
- Volume :
- 57
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Current Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 33937442
- Full Text :
- https://doi.org/10.1007/s00284-008-9194-9