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Antibody–gold quantum dot–PAMAM dendrimer complex as an immunoglobulin immunoassayThe HTML version of this article has been enhanced with colour images.
- Source :
- Analyst; Apr2008, Vol. 133 Issue 5, p667-672, 6p
- Publication Year :
- 2008
-
Abstract
- Gold quantum dots (AuQDs) were synthesized and electrostatically conjugated to goat-derived anti-human IgG for the purpose of detecting human IgG in solution over a broad range of concentrations. The system is able to detect human IgG by linear fluorescence quenching over a micromolar to nanomolar concentration range. We have demonstrated the specificity and a wide dynamic range of the proposed immunoassay. The quenching is a result of competitive surface quenching of the AuQDs. Characterization, details of the immunoassay, and the quenching mechanism, are discussed. [ABSTRACT FROM AUTHOR]
- Subjects :
- IMMUNOGLOBULIN G
IMMUNOLOGY
IMMUNOASSAY
ANTIGENS
Subjects
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 133
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 34951815
- Full Text :
- https://doi.org/10.1039/b718730k