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Antitumor activity and mechanisms of action of total glycosides from aerial part of Cimicifuga dahurica targeted against hepatoma.
- Source :
- BMC Cancer; 2007, Vol. 7, p237-246, 10p
- Publication Year :
- 2007
-
Abstract
- <bold>Background: </bold>Medicinal plant is a main source of cancer drug development. Some of the cycloartane triterpenoids isolated from the aerial part of Cimicifuga dahurica showed cytotoxicity in several cancer cell lines. It is of great interest to examine the antiproliferative activity and mechanisms of total triterpenoid glycosides of C. dahurica and therefore might eventually be useful in the prevention or treatment of Hepatoma.<bold>Methods: </bold>The total glycosides from the aerial part (TGA) was extracted and its cytotoxicity was evaluated in HepG2 cells and primary cultured normal mouse hepatocytes by an MTT assay. Morphology observation, Annexin V-FITC/PI staining, cell cycle analysis and western blot were used to further elucidate the cytotoxic mechanism of TGA. Implanted mouse H22 hepatoma model was used to demonstrate the tumor growth inhibitory activity of TGA in vivo.<bold>Results: </bold>The IC50 values of TGA in HepG2 and primary cultured normal mouse hepatocytes were 21 and 105 mug/ml, respectively. TGA induced G0/G1 cell cycle arrest at lower concentration (25 mug/ml), and triggered G2/M arrest and apoptosis at higher concentrations (50 and 100 mug/ml respectively). An increase in the ratio of Bax/Bcl-2 was implicated in TGA-induced apoptosis. In addition, TGA inhibited the growth of the implanted mouse H22 tumor in a dose-dependent manner.<bold>Conclusion: </bold>TGA may potentially find use as a new therapy for the treatment of hepatoma. [ABSTRACT FROM AUTHOR]
- Subjects :
- BUGBANE
MEDICINAL plants
GLYCOSIDES
HEPATOCELLULAR carcinoma
DRUG development
ANTINEOPLASTIC agents
PROTEIN metabolism
ANIMAL experimentation
APOPTOSIS
CALCIUM-binding proteins
CANCER cells
CELL culture
CELL cycle
CELL physiology
COMPARATIVE studies
DOSE-effect relationship in pharmacology
LIVER tumors
RESEARCH methodology
MEDICAL cooperation
MICE
PROTEINS
RESEARCH
RESEARCH funding
PLANT extracts
EVALUATION research
CANCER cell culture
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 14712407
- Volume :
- 7
- Database :
- Complementary Index
- Journal :
- BMC Cancer
- Publication Type :
- Academic Journal
- Accession number :
- 34963749
- Full Text :
- https://doi.org/10.1186/1471-2407-7-237