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Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells

Authors :
Taura, Daisuke
Noguchi, Michio
Sone, Masakatsu
Hosoda, Kiminori
Mori, Eisaku
Okada, Yohei
Takahashi, Kazutoshi
Homma, Koichiro
Oyamada, Naofumi
Inuzuka, Megumi
Sonoyama, Takuhiro
Ebihara, Ken
Tamura, Naohisa
Itoh, Hiroshi
Suemori, Hirofumi
Nakatsuji, Norio
Okano, Hideyuki
Yamanaka, Shinya
Nakao, Kazuwa
Source :
FEBS Letters; Mar2009, Vol. 583 Issue 6, p1029-1033, 5p
Publication Year :
2009

Abstract

Abstract: Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12days of embryoid body formation and an additional 10days of differentiation on Poly-l-ornithine and fibronectin- coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBPα, PPARγ2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
00145793
Volume :
583
Issue :
6
Database :
Complementary Index
Journal :
FEBS Letters
Publication Type :
Academic Journal
Accession number :
36972378
Full Text :
https://doi.org/10.1016/j.febslet.2009.02.031