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Pseudotyping lentiviral vectors with the wild-type measles virus glycoproteins improves titer and selectivity.
- Source :
- Gene Therapy; May2009, Vol. 16 Issue 5, p700-705, 6p, 1 Chart, 3 Graphs
- Publication Year :
- 2009
-
Abstract
- We pseudotyped HIV-1 vectors with cytoplasmic tail-truncated envelope glycoproteins of a wild-type (WT) measles virus (MV). The particles entered the lymphatic cells exclusively through the signaling lymphocyte activation molecule (SLAM, CD150), whereas particles pseudotyped with the MV vaccine strain glycoproteins also recognized the ubiquitous membrane cofactor protein (CD46) as receptor and had less specific cell entry. MV<subscript>WT</subscript>-HIV vectors reached titers of 10<superscript>8</superscript> t.u. ml<superscript>−1</superscript>, which were up to 10-fold higher than those of MV<subscript>Vac</subscript>-HIV vectors, and discriminated between SLAM-positive and SLAM-negative cells, also in mixed cell cultures. As these vectors transduce primary human cells more efficiently than vesicular stomatitis virus-G pseudotyped vectors do, they are promising candidates for gene transfer to human lymphocytes and certain epithelial cells.Gene Therapy (2009) 16, 700–705; doi:10.1038/gt.2009.11; published online 12 February 2009 [ABSTRACT FROM AUTHOR]
- Subjects :
- MEASLES
GLYCOPROTEINS
VIRUS diseases
PARAMYXOVIRUSES
LYMPHOCYTE transformation
Subjects
Details
- Language :
- English
- ISSN :
- 09697128
- Volume :
- 16
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Gene Therapy
- Publication Type :
- Academic Journal
- Accession number :
- 39664723
- Full Text :
- https://doi.org/10.1038/gt.2009.11