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The peptide-specific alloreactive human T cell repertoire varies largely between individuals and is not extended in HLA-A*0205–anti-HLA-A*0201 pairings.
- Source :
- International Immunology; Jul2001, Vol. 13 Issue 7, p863-870, 8p
- Publication Year :
- 2001
-
Abstract
- Alloreactive T cells recognize framework or peptide-dependent determinants on foreign MHC molecules. Among the peptide-dependent alloreactive T cells a significant proportion is specific for one particular peptide presented by the allo-MHC molecule as antigen-specific T cells would do. Such alloreactive, peptide-specific T cells are referred to as `allorestricted'. High-avidity HLA-A*02 allorestricted cytotoxic T lymphocyte (CTL) clones specific for peptide libraries can be generated from HLA-A*02– donors. We made use of this technique to study the role of closely related self-HLA molecules on shaping of the alloreactive T cell repertoire. Peripheral blood lymphocytes from HLA-A*0205 individuals were stimulated by HLA-A*0201 targets pulsed with an HLA-A*0201 peptide library. We did not observe a bias towards peptide-specific CTL in the HLA-A*0201-directed alloreactive repertoire of HLA-A*0205 donors as compared to HLA-A*02– donors. Comparison of the alloreactive T cell response between two donors having similar HLA haplotypes demonstrated that the allorestricted T cell repertoire is largely different between individuals. [ABSTRACT FROM PUBLISHER]
Details
- Language :
- English
- ISSN :
- 09538178
- Volume :
- 13
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- International Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 44423908
- Full Text :
- https://doi.org/10.1093/intimm/13.7.863