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Optimization and validation of a robust human T-cell culture method for monitoring phenotypic and polyfunctional antigen-specific CD4 and CD8 T-cell responses.
- Source :
- Cytotherapy (Taylor & Francis Ltd); Dec2009, Vol. 11 Issue 7, p912-922, 11p, 7 Graphs
- Publication Year :
- 2009
-
Abstract
- Background aims Monitoring cellular immune responses is one prerequisite for the rational development of cancer vaccines. Methods We describe an extensive effort to optimize and validate quantitatively an in vitro T-cell culture method by determining the phenotype and function of both CD4+ and CD8+ T cells, including measurement of the phenotype markers CCR7, CD45RA, CD28 and CD27 and the functional markers interferon (IFN)-γ, interleukin (IL)-2, macrophage inflammatory protein (MIP)-1β, tumor necrosis factor (TNF)-αand CD107a. Results Autologous peripheral blood mononuclear cells (PBMC) were potent stimulators that expanded antigen (Ag)-specific CD8+ T cells during short-term culture with the addition of IL-2 and IL-15 cytokines. Polyfunctional Ag-specific CD4+ and CD8+ T cells were detectable using this method. Conclusions Our culture system represents a robust human T-cell culture protocol that permits phenotypic, quantitative and qualitative evaluation of vaccine-induced CD4+ and CD8+ T-cell responses. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELL culture
T cells
IMMUNE response
CELLULAR immunity
CYTOKINES
Subjects
Details
- Language :
- English
- ISSN :
- 14653249
- Volume :
- 11
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Cytotherapy (Taylor & Francis Ltd)
- Publication Type :
- Academic Journal
- Accession number :
- 44820824
- Full Text :
- https://doi.org/10.3109/14653240903136987