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Bi-directional activation between mesenchymal stem cells and CLL B-cells: implication for CLL disease progression.
- Source :
- British Journal of Haematology; Nov2009, Vol. 147 Issue 4, p471-483, 13p, 2 Charts, 6 Graphs
- Publication Year :
- 2009
-
Abstract
- It was hypothesized that contact between chronic lymphocytic leukaemia (CLL) B-cells and marrow stromal cells impact both cell types. To test this hypothesis, we utilized a long-term primary culture system from bone biopsies that reliably generates a mesenchymal stem cell (MSC). Co-culture of MSC with CLL B-cells protected the latter from both spontaneous apoptosis and drug-induced apoptosis. The CD38 expression in previously CD38 positive CLL B-cells was up-regulated with MSC co-culture. Upregulation of CD71, CD25, CD69 and CD70 in CLL B-cells was found in the co-culture. CD71 upregulation was more significantly associated with high-risk CLL, implicating CD71 regulation in the microenvironment predicting disease progression. In MSC, rapid ERK and AKT phosphorylation (within 30 min) were detected when CLL B-cells and MSC were separated by transwell; indicating that activation of MSC was mediated by soluble factors. These findings support a bi-directional activation between bone marrow stromal cells and CLL B-cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- LYMPHOCYTIC leukemia
B cells
APOPTOSIS
PHOSPHORYLATION
BONE marrow
Subjects
Details
- Language :
- English
- ISSN :
- 00071048
- Volume :
- 147
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- British Journal of Haematology
- Publication Type :
- Academic Journal
- Accession number :
- 44844105
- Full Text :
- https://doi.org/10.1111/j.1365-2141.2009.07868.x