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The human brain utilizes lactate via the tricarboxylic acid cycle: a 13C-labelled microdialysis and high-resolution nuclear magnetic resonance study.
- Source :
- Brain: A Journal of Neurology; Oct2009, Vol. 132 Issue 10, p2839-2849, 11p, 1 Diagram, 2 Charts, 2 Graphs
- Publication Year :
- 2009
-
Abstract
- Energy metabolism in the human brain is not fully understood. Classically, glucose is regarded as the major energy substrate. However, lactate (conventionally a product of anaerobic metabolism) has been proposed to act as an energy source, yet whether this occurs in man is not known. Here we show that the human brain can indeed utilize lactate as an energy source via the tricarboxylic acid cycle. We used a novel combination of 13C-labelled cerebral microdialysis both to deliver 13C substrates into the brain and recover 13C metabolites from the brain, and high-resolution 13C nuclear magnetic resonance. Microdialysis catheters were placed in the vicinity of focal lesions and in relatively less injured regions of brain, in patients with traumatic brain injury. Infusion with 2-13C-acetate or 3-13C-lactate produced 13C signals for glutamine C4, C3 and C2, indicating tricarboxylic acid cycle operation followed by conversion of glutamate to glutamine. This is the first direct demonstration of brain utilization of lactate as an energy source in humans. [ABSTRACT FROM PUBLISHER]
- Subjects :
- BRAIN physiology
LACTATES
KREBS cycle
MICRODIALYSIS
NUCLEAR magnetic resonance
Subjects
Details
- Language :
- English
- ISSN :
- 00068950
- Volume :
- 132
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Brain: A Journal of Neurology
- Publication Type :
- Academic Journal
- Accession number :
- 45305406
- Full Text :
- https://doi.org/10.1093/brain/awp202