Back to Search
Start Over
Lipid-induced Insulin Resistance Is Associated With Increased Monocyte Expression of Scavenger Receptor CD36 and Internalization of Oxidized LDL.
- Source :
- Obesity (19307381); Dec2009, Vol. 17 Issue 12, p2142-2148, 7p, 1 Chart, 4 Graphs
- Publication Year :
- 2009
-
Abstract
- Elevated free fatty acids (FFAs) contribute to the development of insulin resistance, type 2 diabetes mellitus (T2DM), and may be atherogenic. We tested the relationship among lipid-induced insulin resistance, endothelial dysfunction, and monocyte capacity to form foam cells through scavenger receptor A (SRA) and CD36. Ten healthy subjects underwent 24-h infusion of Intralipid/heparin and saline (0.5 ml/min) on two separate occasions followed by brachial artery reactivity testing and a euglycemic hyperinsulinemic (80 mU/(kg·min)) clamp study to determine insulin sensitivity. Isolation of blood monocytes was performed 24 h after infusion. Surface expression and function of CD36 and SRA to take up oxidized low-density lipoprotein (oxLDL) was determined by flow cytometry and quantitative confocal imaging. Lipid infusion resulted in a twofold increase in serum FFA levels, reduced whole-body glucose disposal by ~20% (P < 0.05), and possibly impaired endothelial-dependent vasodilation (P = 0.1). Blood monocytes obtained during lipid infusion demonstrated a ~25% increase in cell surface expression of CD36 (P < 0.05) but no change in SRA expression. Enhanced CD36 expression was associated with a 50% increase in internalization of oxLDL (P < 0.05). The increase in CD36 surface expression during lipid infusion correlated inversely with glucose disposal (P < 0.05) and not with FFA levels or brachial artery dilation. These data support a role for FFAs in induction of insulin resistance and provide a link to atherogenic mechanisms mediated by expression of scavenger receptor CD36. [ABSTRACT FROM AUTHOR]
- Subjects :
- FATTY acids
INSULIN resistance
TYPE 2 diabetes
LIPIDS
ENDOTHELIUM
Subjects
Details
- Language :
- English
- ISSN :
- 19307381
- Volume :
- 17
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Obesity (19307381)
- Publication Type :
- Academic Journal
- Accession number :
- 45317188
- Full Text :
- https://doi.org/10.1038/oby.2009.179