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T cell receptor beta chain (TCR-Vβ) repertoire of circulating CD4+ CD25−, CD4+ CD25low and CD4+ CD25high T cells in patients with long-term renal allograft survival.
- Source :
- Transplant International; Jan2010, Vol. 23 Issue 1, p54-63, 10p, 1 Chart, 4 Graphs
- Publication Year :
- 2010
-
Abstract
- The mechanisms underlying maintenance of renal allografts in humans under minimal or conventional immunosuppression are poorly understood. There is evidence that CD4<superscript>+</superscript> CD25<superscript>+</superscript> regulatory T cells and clonal deletion, among other mechanisms of tolerance, could play a key role in clinical allograft survival. Twenty-four TCR-Vβ families were assessed in CD4<superscript>+</superscript> CD25<superscript>−</superscript>, CD4<superscript>+</superscript> CD25<superscript>low</superscript> and CD4<superscript>+</superscript> CD25<superscript>high</superscript> T cells from patients with long-term renal allograft survival (LTS), patients exhibiting chronic rejection (ChrRx), patients on dialysis (Dial) and healthy controls (HC) by flow cytometry. LTS patients presented a higher variability in their TCR-Vβ repertoire, such decreased percentage of Vβ2<superscript>+</superscript>, Vβ8a<superscript>+</superscript> and Vβ13<superscript>+</superscript> in CD4<superscript>+</superscript> CD25<superscript>low</superscript> and <superscript>high</superscript> compared with CD4<superscript>+</superscript> CD25<superscript>−</superscript> subset and increased Vβ4 and Vβ7 families in CD4<superscript>+</superscript> CD25<superscript>high</superscript> T cells exclusively. Additionally, LTS patients, particularly those that were not receiving calcineurin inhibitors (CNI), had increased percentages of CD4<superscript>+</superscript> CD25<superscript>high</superscript> T cells when compared with Dial ( P < 0.05) and ChrRx ( P < 0.05) patients. Our results suggest that a differential expression of particular TCR-Vβ families and high levels of circulating CD4<superscript>+</superscript> CD25<superscript>high</superscript> T cells in long-term surviving renal transplant patients could contribute to an active and specific state of immunologic suppression. However, the increase in this T cell subset with regulatory phenotype can be affected by CNI. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09340874
- Volume :
- 23
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Transplant International
- Publication Type :
- Academic Journal
- Accession number :
- 45469379
- Full Text :
- https://doi.org/10.1111/j.1432-2277.2009.00946.x