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Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA.
- Source :
- Nature Methods; Jan2010, Vol. 7 Issue 1, p47-49, 3p, 3 Graphs
- Publication Year :
- 2010
-
Abstract
- Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations. [ABSTRACT FROM AUTHOR]
- Subjects :
- TRANSCRIPTION factors
CHROMATIN
DNA
NUCLEOTIDE sequence
DNA-protein interactions
Subjects
Details
- Language :
- English
- ISSN :
- 15487091
- Volume :
- 7
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Nature Methods
- Publication Type :
- Academic Journal
- Accession number :
- 47163212
- Full Text :
- https://doi.org/10.1038/nmeth.1404