Back to Search Start Over

Comparative proteomic analysis of malformed umbilical cords from somatic cell nuclear transfer-derived piglets: implications for early postnatal death.

Authors :
Jong-Yi Park
Jae-Hwan Kim
Yun-Jung Choi
Kyu-Chan Hwang
Seong-Keun Cho
Ho-Hyun Park
Seung-Sam Paik
Teoan Kim
ChanKyu Park
Hoon Taek Lee
Han Geuk Seo
Soo-Bong Park
Seongsoo Hwang
Jin-Hoi Kim
Source :
BMC Genomics; 2009, Vol. 10, p511-525, 15p, 4 Color Photographs, 1 Chart, 5 Graphs
Publication Year :
2009

Abstract

Background: Somatic cell nuclear transfer (scNT)-derived piglets have high rates of mortality, including stillbirth and postnatal death. Here, we examined severe malformed umbilical cords (MUC), as well as other organs, from nine scNT-derived term piglets. Results: Microscopic analysis revealed complete occlusive thrombi and the absence of columnar epithelial layers in MUC (scNT-MUC) derived from scNT piglets. scNT-MUC had significantly lower expression levels of platelet endothelial cell adhesion molecule-1 (PECAM-1) and angiogenesis-related genes than umbilical cords of normal scNT piglets (scNT-N) that survived into adulthood. Endothelial cells derived from scNT-MUC migrated and formed tubules more slowly than endothelial cells from control umbilical cords or scNT-N. Proteomic analysis of scNT-MUC revealed significant down-regulation of proteins involved in the prevention of oxidative stress and the regulation of glycolysis and cell motility, while molecules involved in apoptosis were significantly up-regulated. Histomorphometric analysis revealed severe calcification in the kidneys and placenta, peliosis in the liver sinusoidal space, abnormal stromal cell proliferation in the lungs, and tubular degeneration in the kidneys in scNT piglets with MUC. Increased levels of apoptosis were also detected in organs derived from all scNT piglets with MUC. Conclusion: These results suggest that MUC contribute to fetal malformations, preterm birth and low birth weight due to underlying molecular defects that result in hypoplastic umbilical arteries and/or placental insufficiency. The results of the current study demonstrate the effects of MUC on fetal growth and organ development in scNT-derived pigs, and provide important insight into the molecular mechanisms underlying angiogenesis during umbilical cord development. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14712164
Volume :
10
Database :
Complementary Index
Journal :
BMC Genomics
Publication Type :
Academic Journal
Accession number :
47217310
Full Text :
https://doi.org/10.1186/1471-2164-10-511