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A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2′-Deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in Plants.
- Source :
- Plant & Cell Physiology; Nov2009, Vol. 50 Issue 11, p1988-1993, 6p, 4 Graphs
- Publication Year :
- 2009
-
Abstract
- A highly sensitive quantitative method for assaying nicotianamine (NA) and 2′-deoxymugineic acid (DMA) using liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) was developed. The amino and hydroxyl groups of NA and DMA were derivatized using 9-fluorenylmethoxycarbonyl chloride. The amounts of NA and DMA in 10 μl of xylem sap from rice cultivated under iron (Fe)-sufficient and Fe-deficient conditions were quantified without concentration. In Fe-sufficient plants, the concentrations of NA and DMA were almost equal to that of Fe. In Fe-deficient plants, the concentration of NA did not change significantly, whereas that of DMA increased markedly. [ABSTRACT FROM PUBLISHER]
Details
- Language :
- English
- ISSN :
- 00320781
- Volume :
- 50
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Plant & Cell Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 47429919
- Full Text :
- https://doi.org/10.1093/pcp/pcp141